UPLC fingerprint of xanthii fructus from different habitats.
- Author:
Yan HONG
1
;
Yan-Quan HAN
;
Lun-Zhu XIA
;
Jie GUI
;
Xi CHEN
;
Yan-Hua SUN
Author Information
1. School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230031, China. hyan2003@163.com
- Publication Type:Journal Article
- MeSH:
China;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
analysis;
Ecosystem;
Fruit;
chemistry;
Quality Control;
Xanthium;
chemistry
- From:
China Journal of Chinese Materia Medica
2013;38(11):1766-1771
- CountryChina
- Language:Chinese
-
Abstract:
This study was establish an UPLC fingerprint of Xanthii Fructus from different habitats, to provide a comprehensive evaluation for its quality control. UPLC-PDA was adopted to analysis of 26 baches of Xanthii Fructus from different habitats. The chromatographic condition was as follow: ACQUITY BEH C18 Column (2.1 mm x 100 mm,1.7 microm) eluted with the mobile phases of acetonitrile and 0.1% phosphoric acid water in gradient mode. The flow rate was 0.25 mL x min(-1) and the detection wavelength was set at 220 nm. The fingerprints of 26 batches Xanthii Fructus were carried out by similarity comparation, cluster and the principal component analysis (PCA). There were nineteen common peaks, nine of which had been identified, and the similarity degrees of the twenty-six batches of the samples were between 0.804 and 0.990. All the samples were classified into six categories, and the PCA value of each fingerprint peak was calculated, and six principal components accounted for over 81. 140% of the total variance were extracted from the original data This method can be used to assess the quality of Xanthii Fructus.
