Screening and identification of differential expression genes related to stress fracture by cDNA microarray assay.
- Author:
Hua-bing ZHAO
1
;
Yi-zheng WANG
;
Xiao-xia LAN
;
Ming-shun ZHANG
;
Guo-ping ZHAO
;
Guang-ya YIN
;
Shi-xin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Case-Control Studies; DNA, Complementary; genetics; Fractures, Stress; blood; metabolism; GPI-Linked Proteins; genetics; metabolism; Gene Expression; Gene Expression Profiling; Humans; Male; Military Personnel; Oligonucleotide Array Sequence Analysis; Receptors, Tumor Necrosis Factor, Member 10c; Tumor Necrosis Factor Decoy Receptors; genetics; metabolism; Young Adult
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(11):827-830
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the differentially expressed genes between the Stress fracture (SF) cases and controls.
METHODSTotal RNA was extracted and purified from peripheral blood sample of 3 SF cases and 3 controls who conducted a 1:1 matched case-control study, then used for Human Genome Array analysis. The hybridization data were analyzed using SAM software. Parts of these genes were analyzed and identified by real-time PCR.
RESULTSUpregulated and downregulated genes were 22 and 1, respectively. Thus the highest ratio and most significant cytokine was tumor necrosis factor receptor superfamily, member 10c (TNFRSF10C). The result of real-time PCR shows that TNFRSF10C was over-expressed in 3 cases and low-expressed in 1 case.
CONCLUSIONObvious difference exists in gene expression between SF cases and controls, showing there may be a lot of genes involving in the occurrence and development of SF. Meanwhile, the identification of the specific genes is helpful for biomechanics study, early diagnosis and screening of SF.
