Induction of cell damage and change of miR-133b expression by paraquat in PC12 cells.
- Author:
Huang-yuan LI
1
;
Si-ying WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; MicroRNAs; metabolism; PC12 Cells; Paraquat; toxicity; Rats
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(1):2-6
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of paraquat on induction of cell damage and miR-133b expression in PC12 cells.
METHODSCytotoxicity of PC12 cells was measured by MTT assay, following the PC12 cells treatment with 50, 100, or 300 µmol/L paraquat. Cell apoptosis was examined by the method of Annexin V-FITC/PI in flow cytometry (FCM) and the relative level of miR-133b expression was measured by real time RT-PCR, following the PC12 cells treatment with 100 or 300 µmol/L paraquat.
RESULTSSurvival rate of PC12 cells treated with 100 or 300 µmol/L paraquat was lower than that of the vehicle control group (P < 0.01, P < 0.05), in the dose dependent pattern. Apoptotic rate of PC12 cells treated with 100, 300 µmol/L paraquat was higher than that of the vehicle control group (P < 0.05). The relative level of miR-133b expression of PC12 cells treated with 300 µmol/L paraquat was higher than that of the vehicle control group (P < 0.05).
CONCLUSIONSParaquat may cause cell damage and induce apoptosis in PC12 cells, and induce miR-133b expression.
