The influence of SiO2 on epithelial-mesenchymal transition (EMT) in human bronchial epithelial cells.
- Author:
Guan-nan LIANG
1
;
Jian-hua ZHOU
;
Yong-bin HU
;
Xiang LI
;
Zhen-qin GAO
;
Hai-ying JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Bronchi; cytology; Cells, Cultured; Epithelial Cells; cytology; drug effects; Epithelial-Mesenchymal Transition; drug effects; Humans; Silicon Dioxide; adverse effects; Stromal Cells; cytology; drug effects
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(1):7-10
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate SiO2-induced EMT in human bronchial epithelial cells HBE in vitro.
METHODSHBE cells were cultured and then stimulated with indicated doses of SiO2 (0, 50, 100, 200, 300 µg/ml). The morphological changes were observed by microscope. In addition, Western blot was per-formed to detect the expression of E-cad, α-SMA and Vim. The changes of migration ability were examined by wound-healing assay in vitro.
RESULTS(1) After exposure to SiO2, HBE cells lost contact with their neighbor and displayed a spindle-shape, fibroblast-like morphology. (2) Compared with the control, the E-cad (300 µg/ml group) expression downregulated 2.98 fold (P < 0.05), and the Vim (300 µg/ml group) and α-SMA (200 µg/ml group) expression upregulated 4.46 fold and 3.55 fold (P < 0.05). There were significant differences between 100, 200, 300 µg/ml groups and the control group (P < 0.05). (3) In the test group, the percentage of wound-healing areas/wound areas were larger than those in control group (P < 0.05).
CONCLUSIONSSiO2 could induce EMT in human bronchial epithelial cells.
