High-resolution melting: a analysis for genotyping of MDR1 C3435T in benzene-exposed workers.
- Author:
Jian-shu HUANG
1
;
Xin-ju ZHANG
;
Xiao XU
;
Ming GUAN
;
Yuan-ling ZHOU
;
Ling LÜ
;
He-jian ZOU
Author Information
- Publication Type:Journal Article
- MeSH: ATP Binding Cassette Transporter, Sub-Family B; ATP-Binding Cassette, Sub-Family B, Member 1; genetics; Benzene; Genotype; Genotyping Techniques; methods; Heterozygote; Humans; Occupational Exposure; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(1):70-72
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEUsing high resolution melting (HRM) to analysis MDR1 C3435T in people exposed to benzene.
METHODSRestriction fragment length polymorphism (RFLP) was utilized to detect the polymorphism of MDR1 3435 in 121 benzene-exposed workers, and the results were compared with the HRM in 10% samples and were confirmed with direct sequencing for six people in them.
RESULTSBy direct sequencing, consistent results of benzene-exposed workers with RFLP or HRM were got. The new high resolution melting curve analysis is more efficient, more convenient, and cheaper than RFLP.
CONCLUSIONHigh-resolution melting analysis provides a valid approach to efficiently detect DNA genetic diagnosis, which is suitable for detect susceptible genes in occupational surveillance.
