Determination of epimedin C and icariin in Herba Epimedii by HPLC.
- Author:
Ming-quan WANG
1
;
Zhi-ming BI
;
Ping LI
;
Hui JI
;
Fei-long CHENG
Author Information
- Publication Type:Journal Article
- MeSH: China; Drugs, Chinese Herbal; analysis; Ecosystem; Epimedium; chemistry; classification; Flavonoids; analysis; Plant Components, Aerial; chemistry; Plants, Medicinal; chemistry; Quality Control; Species Specificity
- From: China Journal of Chinese Materia Medica 2003;28(11):1025-1027
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RP-HPLC method for the determination of epimedin C and icariin in Herba Epimedii.
METHODChromatographic conditions: Hypersil BDS-C18 column, acetonitrice and water containing 0.05% phosphoric acid as gradient eluents, G1315A photodiode array detector at 272 nm.
RESULTThe average recovery rate of epimedin C was 99.7%, RSD 1.5% (n = 9), icariin 102.5%, RSD 1.1% (n=9).
CONCLUSIONThe method is reliable, stable and well repeatable. It provides the useful information for quality evaluation.
