Determination of concentration and encapsulation efficiency of silybin liposomes.
- Author:
Jiang-nan YU
1
;
Xi-ming XU
;
Yuan ZHU
;
Hao GAO
Author Information
- Publication Type:Journal Article
- MeSH: Drug Delivery Systems; Liposomes; chemistry; Milk Thistle; chemistry; Plants, Medicinal; chemistry; Quality Control; Silymarin; administration & dosage; analysis; isolation & purification; Spectrophotometry, Ultraviolet; Technology, Pharmaceutical; methods
- From: China Journal of Chinese Materia Medica 2003;28(11):1027-1030
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the encapsulation efficiency of silybin liposomes.
METHODAfter Silybin liposomes solution was separated through sphadex G-100 column, eluent collected was determined by the first derivative UV spectrometry.
RESULTThe calibration curve of Silybin was linear in the range of 0.5-30.0 mg x L(-1) (r = 0.9998). The average recovery was 101.7%. The relative standard deviation was 1.8%. The results showed that the encapsulation efficiency ranged from 65.1%-83.0% when the drug/phospholipid ratio varied from 0.02-0.06. The concentration of SLB in liposomes prepared by optimum technique was 760.4 mg x L(-1) (n = 4).
CONCLUSIONThe method is accurate, simple and suitable for the quality control of Silybin liposomes.
