Study of the methods of determining the isoflavones in herbs of Trifolium pratense extractive.
- Author:
Qing-quan BIAN
1
;
Zhen-ping YANG
;
Mei-zhong MA
;
Song LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; isolation & purification; Hydrolysis; Isoflavones; analysis; chemistry; Plants, Medicinal; chemistry; Quality Control; Trifolium; chemistry
- From: China Journal of Chinese Materia Medica 2003;28(11):1044-1047
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the methods of determining the amount of Isoflavones in red clover extractive.
METHODRP-HPLC is employed to determine the Isoflavones, with a C18 RP Column, a moble phase of MeOH-CH3CN-0.1%H3PO4 and a detection wavelength of 260 nm.
RESULTAfter being water-solved, the four groups of flavoes elements satisfactorily separated, the amount of feeding in a range of 0.024-0.336 microg which has a good linear relationship with the peaks, and the total isoflavones determining results repetition, RSD=10.1%.
CONCLUSIONA simple, reliable and effective quality-control method is given.
