The effects of si-wu-tang on serum protein of blood deficient mice induced by radiation.
- Author:
Zeng-chun MA
1
;
Yue GAO
;
Hong-ling TANG
;
Sheng-qi WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Autoantigens; blood; Carrier Proteins; Cytoskeletal Proteins; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Dystonin; Dystrophin; blood; Female; Kinesin; blood; Mice; Mice, Inbred C57BL; Nerve Tissue Proteins; Non-Fibrillar Collagens; blood; Plants, Medicinal; chemistry; Radiation Injuries, Experimental; blood; Radiation-Protective Agents; pharmacology; Whole-Body Irradiation
- From: China Journal of Chinese Materia Medica 2003;28(11):1050-1053
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of Si-Wu-Tang on serum protein of blood deficient mice b y proteomicstechnique and study the enriching and regulating blood mechanism of Si-Wu-Tang on mocular level.
METHODThe blood deficient mice was induced by using a single dose of 3.5 Gy radiation from a 60Cogamma source, and high resolution two-dimensional polyacryamide gel electrophoresis (2-DE), computer-assisted image analysis, and mass spectrometry were used to detect regulated protein by Si-Wu-Tang.
RESULT12 lower and 4 higher protein in sera could be recovered by Si-Wu-Tang, 4 protein might be DNA-dependent protein kinase catalytic subunit, Dystrophin, KIF13A, dystonin. They play a part in DNA double-stranded break repair, recombination and modulation of transcription, transportation of mannose-6-phosphate receptor, etc.
CONCLUSIONSi-Wu-Tang can regulate serum protein in blood deficient mice, resulting in improving hematopoiesis and lessening irradiated injury.
