The effect of jiunaoyizhi capsul on signal transduction pathway of SY5Y cell lines.
- Author:
Yan MENG
1
;
Jing-yan ZHANG
;
Rong WANG
;
Zhi-juan JI
;
Shu-li SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; drug effects; Cyclic AMP Response Element-Binding Protein; metabolism; Cytochromes c; metabolism; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Humans; Neuroblastoma; metabolism; pathology; Neurons; drug effects; Plants, Medicinal; chemistry; Signal Transduction
- From: China Journal of Chinese Materia Medica 2003;28(11):1076-1078
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Jiunaoyizhi capsul on the signal transduction pathway of SY5Y cell lines and explore the mechanism of the function of the capsul's enhancing neuronal growth.
METHODHuman neuroblastoma was used as cell models and they were divided into control group and experimental group. Supernatant of cell lysate was taken and immunoprecipitation was done with antibodies to proteins related to signal transduction pathway, and the immunoprecipitates were analyzed by Western blotting.
RESULTAfter treatment with Jiunaoyizhi capsul, expression of Akt/PKB, CREB, P-CREB was clearly increased and expression of cytochrome C decreased more than the control group.
CONCLUSIONJiunaoyizhi capsul can promote expression of some proteins related with signal transduction pathway in SY5Y cell lines. Mechanism of Jiunaoyizhi capsul's enhancing neuronal growth is relevant to expression of some proteins in signal transduction pathway.
