Extraction and characterization of total DNA from Dendrobium.
- Author:
Rui PENG
1
;
Hong-yuan SONG
;
Quan-sen LI
;
Yu WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cetrimonium Compounds; DNA, Plant; isolation & purification; Dendrobium; classification; genetics; Electrophoresis; Plants, Medicinal; classification; genetics; Quality Control; Random Amplified Polymorphic DNA Technique; Spectrophotometry, Ultraviolet
- From: China Journal of Chinese Materia Medica 2003;28(12):1129-1131
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the quality of DNA with three methods of DNA extraction and the influence on RAPD-PCR.
METHODthe electrophoresis of total DNA, UV spectrophotometry and RAPD analysis were carried out on DNA extracted from three different methods.
RESULTThe DNA concentration and yields were different, which greatly influenced the result of RAPD-PCR.
CONCLUSIONThe higher quality DNA from Dendrobium can be obtained with the method of CTAB-free extraction medium before total DNA was isolated.
