Determination of four alkaloids in Berberis plants by HPLC.
- Author:
Duo-long DI
1
;
Ye-wei LIU
;
Zhi-gang MA
;
Sheng-xiang JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; analysis; Benzylisoquinolines; analysis; Berberine; analogs & derivatives; analysis; Berberine Alkaloids; analysis; Berberis; chemistry; classification; China; Chromatography, High Pressure Liquid; methods; Ecosystem; Plant Bark; chemistry; Plant Roots; chemistry; Plant Stems; chemistry; Plants, Medicinal; chemistry; classification; Species Specificity
- From: China Journal of Chinese Materia Medica 2003;28(12):1132-1134
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to assess Berberis, a simple and effective high-performance liquid chromatographic (HPLC) method was established.
METHODBy applying HPLC with gradiation elution, this method was developed for determination of four kinds of alkaloids including berbamine, Jatrorrhizine, berberine and palmatine in methanolic extracts from root bark, root, stem bark and stem of Berberis.
RESULTThe alkaloids berbamine, jatrorrhizine, berberine and palmatine shown good linear correlations in the range of 0.028-4.74 microg (r = 0.9998); 0.012-2.0 microg (r = 0.9996); 0.026-0.52 microg (r = 0.9999); 0.015-2.56 microg (r = 0.9998), respectively. The correlation coefficients of the calibration curve for the analytes exceeded 0.9998.
CONCLUSIONThe optimized HPLC method was applied to analyze various samples, and the results shown that the content of alkaloids were obvious difference in Berberis from different area, different species and different parts.
