Effect of factors on callus biomass and synthetic mass of hypericin in Hypericum perforatum.

Author: Ming-shu XU ¹ ; Lu-qi HUANG ; Mei-lan CHEN ; He-chun YE ; Guo-feng LI
Author Information

1. Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Publication Type:Journal Article
MeSH: Culture Media; Hypericum; growth & development; metabolism; Mannose; pharmacology; Nitrogen; pharmacology; Perylene; analogs & derivatives; metabolism; Plants, Medicinal; growth & development; metabolism; Povidone; analogs & derivatives; pharmacology; Tissue Culture Techniques
From: China Journal of Chinese Materia Medica 2003;28(10):921-923
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the effect of several factors on the quantity of hypericin in H. perforatum callus.
METHODHigh efficiency liquid phase chromatography and plant tissue culture were applied.
RESULT AND CONCLUSIONWhen the ratio of nitro-nitrogen to amina-nitrogen is 3:1, the callus biomass is 1.6-fold and the synthetic mass of hypericin rises. 0.1-0.20 mg x L(-1) mannose improves the content of total hypericin. The addition of PVP or PVPP can promote improvement of the growth and biosynthesis of callus.