Effects of extracts of ginkgo biloba (EGB) on levels of nitric oxide and apoptosis in the retina induced by glutamate in adult rabbits.
- Author:
Yang-jun LI
1
;
Xin-guang YANG
;
Mei-rui GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Drugs, Chinese Herbal; isolation & purification; pharmacology; Female; Ginkgo biloba; chemistry; Glutamic Acid; Male; Nitric Oxide; metabolism; Plant Leaves; chemistry; Plants, Medicinal; chemistry; Rabbits; Random Allocation; Retina; cytology; metabolism
- From: China Journal of Chinese Materia Medica 2003;28(10):961-964
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of extracts of ginkgo biloba (EGB) on the levels of nitric oxide (NO) and apoptosis in the retina induced by glutamate by intravitreal injection in adult rabbit.
METHODThe model of apoptosis in retina induced by glutamate by intravitreal injection was established in adult rabbit and EGB was retrobulbarly injected. The levels of NO were measured by spectrophotometer. Retina DNA fragmentation was determined by agarose gel electrophoresis.
RESULTThe levels of NO in retina in experiment groups were significantly increased compared with controls, after treatment with high dosage of EGB, and levels of NO was decreased to normal. They were not decreased by injection of small dosage of EGB. DNA fragmentation of retina apoptosis was detected in experiment groups and small dosage EGB groups.
CONCLUSIONThe retina apoptosis was induced by glutamate by intravitreal injection in adult rabbit and was inhibited by EGB that may be through blocking the generation of NO free radicals.
