Studies on fingerprinting of Flos Buddleja by RP-HPLC.
- Author:
Peng HAN
1
;
Ya-jun CUI
;
Hong-zhu GUO
;
De-an GUO
Author Information
- Publication Type:Journal Article
- MeSH: Buddleja; chemistry; China; Chromatography, High Pressure Liquid; methods; Ecosystem; Flowers; chemistry; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2004;29(10):938-966
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish fingerprinting of Flos Buddleja by using RP-HPLC for the quality control.
METHODThe HPLC condition was as follows: Inertsil ODS-3 C18 analytical column (4.6 mm x 250 mm, 5 microm), gredient eluation with MeCN (0.1% TFA)-H2O (0.1%TFA), flow rate 1.0 mL x min(-1), detection wavelength 254 nm. 10 commercial samples were analyzed to establish a fingerprinting.
RESULTAmong the obtained fingerprinting, most of the detected peaks were separated effectively. The accuracy, repeatability and stability of this method were satisfied. The RSDs of relative retention time and area of aimed peaks which existed in all samples wereless than 5%. Theresults were in accordance with the request of fingerprinting.
CONCLUSIONThe established fingerprinting can be used for the quality control of Flos Buddleja.
