Determination of isoimperatorin and osthol in ganmao yixiaoshi capsule by RP-HPLC.
- Author:
Yue SHI
1
;
Wei-hua WANG
;
Li-jun DU
Author Information
- Publication Type:Journal Article
- MeSH: Angelica; chemistry; Apiaceae; chemistry; Capsules; Chromatography, High Pressure Liquid; methods; Common Cold; Coumarins; analysis; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; Furocoumarins; analysis; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2004;29(10):950-952
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for the determination of isoimperatorin and osthol in Ganmao Yixiaoshi capsule by RP-HPLC.
METHODThe chromatographic conditions included Hypersil C18 column and the mobile phase consisting of a mixture of acetonitrile-water (42.5:57.5). The detection wavelength was at 320 nm.
RESULTThe calibration curve was linear over the concentration range of 0-0.3975 microg for isoimperatorin and 0-0.3825 microg for osthol respectively. The correlation coefficient was r = 0.9999 and r = 0.9997. The average recovery of isoimperatorin was 98.1% and that of osthol was 99.8%.
CONCLUSIONThe method sensitive, simple and accurate for the determination of isoimperatorin and osthol concentrations in Ganmao Yixiaoshi capsule.
