Determination of protopine and isocorydine in root of Dactylicapnos scandens by HPLC.
- Author:
Tian-qing YAN
1
;
Yan-fang YANG
;
Tie-min AI
Author Information
- Publication Type:Journal Article
- MeSH: Aporphines; analysis; Benzophenanthridines; Berberine Alkaloids; analysis; Chromatography, High Pressure Liquid; Papaveraceae; chemistry; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2004;29(10):961-963
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC method for determination of protopine and isocorydine in root of Dactylicapnos scandens.
METHODThe separation was performed in a PHENOMENEX-C18 column with a mobile phase of methanol-0.2% phosphoric acid (adjusted to pH 7.0 with triethylamine)(50:50), The detection wavelength was at 254 nm and the flow rate was 0.8 mL x min(-1).
RESULTThe average recovery of Protopine and Isocorydine was 97.9%, 98.6% respectively, and RSD 1.3%, 1.4%.
CONCLUSIONThis method is accurate, simple and reliable. It can be used for quality control of D. scandens.