Detection of differentially expressed genes in hepatocellularcarcinoma cells SMMC-7721 treated with Typhonium giganteum extract by mRNA differential display.
- Author:
Shun-qi WANG
1
;
Hong NI
;
Hua CHENG
;
Guang-liang WANG
;
Tong-shun WANG
;
Li CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Araceae; chemistry; Carcinoma, Hepatocellular; genetics; pathology; Cell Line, Tumor; Drugs, Chinese Herbal; isolation & purification; pharmacology; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; drug effects; Humans; Liver Neoplasms; genetics; pathology; Plants, Medicinal; chemistry; RNA, Neoplasm; genetics; Rhizome; chemistry
- From: China Journal of Chinese Materia Medica 2004;29(10):974-977
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen and identify the differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responsing to the aqueous extract from dried powdered rhizomes of Typhonium giganteum (AEoTGE).
METHODThe response of hepatocellular carcinoma cells SMMC-7721 to AEoTGE was explored with the technique of mRNA differential display.
RESULTAfter hepatocarcinoma cells SMMC-7721 were treated by AEoTGE for 36 hours, 1 gene expression was upgrade and 1 gene expression was downgrade induced by AEoTGE.
CONCLUSIONThe research has provided important clues for the molecular mechanism of how hepatocarcinoma cells responseing to T. giganteum.
