Endothelium-independent vasorelaxant effect of puerarin on rat thoracic aorta.
- Author:
Kan DONG
1
;
Qian-min TAO
;
Qiang XIA
;
Qi-xian SHAN
;
Guo-biao PAN
Author Information
- Publication Type:Journal Article
- MeSH: 4-Aminopyridine; pharmacology; Animals; Aorta, Thoracic; drug effects; physiology; Endothelium, Vascular; physiology; In Vitro Techniques; Isoflavones; isolation & purification; pharmacology; Male; Phenylephrine; antagonists & inhibitors; Plants, Medicinal; chemistry; Potassium Channel Blockers; pharmacology; Potassium Channels; drug effects; Pueraria; chemistry; Rats; Rats, Sprague-Dawley; Tetraethylammonium; pharmacology; Vasoconstriction; drug effects; Vasodilation; drug effects; Vasodilator Agents; pharmacology
- From: China Journal of Chinese Materia Medica 2004;29(10):981-984
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the vasorelaxant effect of puerarin in rat aortic rings and the mechanism.
METHODThe isolated thoracic aortic rings of male Sprague-Dawley rats were mounted on the organ bath and the contractile responses of the vessel were recorded.
RESULTPuerarin completely relaxed the contractions induced by phenylephrine in a concentration-dependent manner in endothelium-intact and endothelium-denuded rat aorta, but it had no effect on those preconstricted by a high concentration of potassium chloride (KCl, 60 mmol x L(-1)). The relaxant effect of puerarin was significantly inhibited by pretreatment of endothelium-denuded aorta with potassium channel antagonists tetraethylammonium, 4-aminopyridine but not glibenclamide.
CONCLUSIONPuerarin induces an endothelium-independent relaxation in rat aortic rings. The mechanisms may involve the reduction in Ca2+ influx through the calcium channels operated by alpha-adrenergic receptor and the activation of the potassium channels (Kv and BKca, but not KATP).
