Determination of carvacrol and thymol in Mosla chinensis by HPLC.
- Author:
Li JI
1
;
Fang WANG
;
Yuan-yan LIU
;
Yan TONG
;
Xian-duan LI
;
Xue-feng FENG
;
Lu-qi HUANG
;
Guo-ping ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Lamiaceae; chemistry; Monoterpenes; analysis; Plant Components, Aerial; chemistry; Plants, Medicinal; chemistry; Quality Control; Thymol; analysis
- From: China Journal of Chinese Materia Medica 2004;29(11):1030-1032
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a quantitative method of determination of carvacrol and thymol in Mosla chinensis.
METHODThe sample was extracted with 95% ethanol, ODS column was used with methanol-water-acetic acid (60:40:2) as mobile phase. The detection wavelength was set at 274 nm.
RESULTThe linearities of carvacrol and thymol were respectively in the range of 0.23-2.15 microg (r = 0.9999) and 0.39-2.36 microg (r = 0.9999); the average recoveries were 99.9% (RSD 1.4%) and 98.6% (RSD 1.3%); the RSD of repeatability were 1.1% and 1.6%.
CONCLUSIONThe method is reliable, and can be used for quality control of M. chinensis.
