Genetic analysis of cultivated ginseng population with the assistance of RAPD technology.
- Author:
Ai-juan SHAO
1
;
Xin LI
;
Lu-qi HUANG
;
Jian-he WEI
;
Shu-fang LIN
Author Information
- Publication Type:Journal Article
- MeSH: Cluster Analysis; DNA Primers; genetics; DNA, Plant; genetics; Genetic Variation; Panax; classification; genetics; growth & development; Phylogeny; Plants, Medicinal; genetics; growth & development; Random Amplified Polymorphic DNA Technique; methods
- From: China Journal of Chinese Materia Medica 2004;29(11):1033-1036
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo provide reference for breeding of cultivated ginseng by researching the genetic diversities among different strains.
METHODTo make up the systematic diagram of genetic relationship by calculating the proximity coefficient with SPSS 10.0 software and clustered by between-groups linkage method.
RESULTThe selected 18 random primers used in PCR amplification to produce 145 bands in 17 samples, among which 53 bands (36.5%) are polymorphic. The results of cluster analysis show that the genetic relationship between different strains of Da-maya is closer than that of Er-maya, which proves that it is feasible to differentiate the cultivated groups in molecular level on outside character.
CONCLUSIONRAPD is a favorable molecular marker to assist ginseng breeding.
