Determination of orientin and vitexin in Trollius chinesis preparation by HPLC.
- Author:
Zhi LIU
1
;
Li WANG
;
Wen LI
;
Yong HUANG
;
Zu-chao XU
Author Information
- Publication Type:Journal Article
- MeSH: Apigenin; analysis; Capsules; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; administration & dosage; chemistry; Flavonoids; analysis; Flowers; chemistry; Glucosides; analysis; Plants, Medicinal; chemistry; Quality Control; Ranunculaceae; chemistry; Tablets
- From: China Journal of Chinese Materia Medica 2004;29(11):1049-1051
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC method for determining orientin and vitexin in Trollius chinesis preparation.
METHODThe separation was carried out on Hypersil ODS column with a mixture of acetonitrile-1% acetic acid solution (15:85) as the mobile phase, at a flow rate of 1.0 mL x min(-1). The detection wavelength was set 340 nm.
RESULTOrientin showed a good linear relationship in the range of 8.42-84.2 microg x mL(-1) (r = 0.9999) and the average recovery is 98.3%, RSD is 0.8%. Vitexin showed a good linear relationship in the range of 8.07-80.7 microg x mL(-1) (r = 0.9999) and the average recovery is 102.4%. RSD is 1.7%.
CONCLUSIONThe method is sensitive, specific, and accurate with a good repeatability and can be used for the quality control of Trollius chinesis preparation.
