Different expression of cytokines induced by Actinobacillus actinomycetemcomitans lipopolysaccharide in monocytes/macrophages from different organs of rabbits
10.3760/cma.j.issn.1002-0098.2013.03.008
- VernacularTitle:伴放线放线杆菌脂多糖诱导下兔不同部位单核巨噬细胞细胞因子表达的差异
- Author:
Min HUANG
1
;
Hou-Xuan LI
;
Lan LUO
;
Shuai CHEN
;
Yan-Fen LI
;
Fu-Hua YAN
Author Information
1. 福建医科大学附属口腔医院福建省高校口腔医学重点实验室
- Keywords:
Actinobacillus actinomycetemcomitans;
Lipopolysaccharides;
Mononuclear phagocyte;
Cytokines
- From:
Chinese Journal of Stomatology
2013;48(3):155-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of cytokines induced by Actinobacillus actinomycetemcomitans lipopolysaccharide(Aa-LPS) in monocytes/macrophages from different organs of rabbits.Methods The peripheral mononuclear cells(Mo),alveolar macrophages (AM),peritoneal macrophages(PM) and Kupffer cells (KC) from five New Zealand rabbits were isolated respectively.Then the cells from different organs were stimulated with Escherichia coli (Ec)-LPS or Aa-LPS at the dose of 1 mg/L.Ater culture for 24 hours,the expression of tumor necrosis factor-α (TNF-α),interleukin (IL) 6,IL-1β,IL-8 mRNA and protein were determined by real-time PCR and enzyme-linked immunosorbent assay respectively.Results The monocytes/macrophages challenged by Ec-LPS or Aa-LPS expressed more cytokines both in mRNA and protein levels compared with the controls (P ＜ 0.05).Among them,AM displayed the highest respond when encount with Aa-LPS,with the TNF-α,IL-6,IL-1β,1L-8 mRNA relative levels were(0.4719 ±0.0171),(2.7895 ± 0.0669),(5.1527 ±0.1190),(3.6785 ±0.1836) and the proteins concentrations were (82.2 ±5.4),(40.2 ±2.0),(50308.3 ±445.0),(35305.3 ± 1480.9) ng/L respectively.And the inducibility of Aa-LPS was stronger than that of Ec-LPS (P ＜ 0.05).Meanwhile the cells from different organs showed discrepant response when exposed to Aa-LPS (P ＜ 0.05).The results showed their abilities to secrete cytokines were in the sequence of AM ＞ Mo ＞ KC ＞ PM.Conclusions Aa-LPS enfluenced the expression of cytokines in monocytes/macrophages from different organs of rabbits.
