Effect of platelet-rich plasma on cell proliferation and osteogenic activity of pulp stem cells.
- Author:
Sheng-nan ZHAO
1
;
Wen-fei LIU
;
Zhen-ting ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Cell Differentiation; Cell Proliferation; Cells, Cultured; Child; Dental Pulp; cytology; Humans; Male; Middle Aged; Osteogenesis; Platelet-Rich Plasma; Stem Cells; cytology; Young Adult
- From: Chinese Journal of Stomatology 2013;48(3):177-182
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effects of different concentrations of platelet-rich plasma (PRP) on human dental pulp stem cells (hDPSC) proliferation and osteogenic differentiation activity so as to provide basis for future application of dental pulp stem cells and PRP in tissue engineering and bone repair therapy.
METHODShDPSC were isolated and cultivated in vitro. Flow cytometric analysis was carried out to test the expression of STRO-1.hDPSC were cultured in various concentrations of PRP (1%, 5%, 10%, 20%). At the 2nd and 6th day 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was tested. Osteogenic differentiation of hDPSC was assessed using alkaline phosphatase (ALP) staining and Alizarin Red staining at the 7th and 14th day.
RESULTSFlow cytometric analysis demonstrated that 14.82% of hDPSC were STRO-1 positive. One percent to 20% PRP showed significant effect of promoting hDPSC proliferation. One percent to 10% PRP showed significant effect of promoting hDPSC osteogenic differentiation.
CONCLUSIONSCertain concentrations of PRP can promote hDPSC proliferate and osteogenic differentiate, and this finding suggests future application of dental pulp stem cells and PRP in bone tissue engineering.
