Effect of platelet-rich plasma on cell proliferation and osteogenic activity of pulp stem cells
10.3760/cma.j.issn.1002-0098.2013.03.013
- VernacularTitle:富血小板血浆对牙髓干细胞增殖及成骨活性的影响
- Author:
Sheng-Nan ZHAO
1
;
Wen-Fei LIU
;
Zhen-Ting ZHANG
Author Information
1. 100050,首都医科大学口腔医学院修复科
- Keywords:
Platelet-rich plasma;
Cell proliferation;
Cell differentiation;
Dental pulp stem cells
- From:
Chinese Journal of Stomatology
2013;48(3):177-182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of different concentrations of platelet-rich plasma (PRP) on human dental pulp stem cells(hDPSC) proliferation and osteogenic differentiation activity so as to provide basis for future application of dental pulp stem cells and PRP in tissue engineering and bone repair therapy.Methods hDPSC were isolated and cultivated in vitro.Flow cytometric analysis was carried out to test the expression of STRO-1.hDPSC were cultured in various concentrations of PRP(1％,5％,10％,20％).At the 2nd and 6th day 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was tested.Osteogenic differentiation of hDPSC was assessed using alkaline phosphatase (ALP) staining and Alizarin Red staining at the 7th and 14th day.Results Flow cytometric analysis demonstrated that 14.82％ of hDPSC were STRO-1 positive.One percent to 20％ PRP showed significant effect of promoting hDPSC proliferation.One percent to 10％ PRP showed significant effect of promoting hDPSC osteogenic differentiation.Conclusions Certain concentrations of PRP can promote hDPSC proliferate and osteogenic differentiate,and this finding suggests future application of dental pulp stem cells and PRP in bone tissue engineering.
