Effect of mineral trioxide aggregate on proliferation and differentiation of rat dental papilla cells.
- Author:
Fei-lai LI
1
;
Guo-ying QUE
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Aluminum Compounds; administration & dosage; pharmacology; Animals; Calcium Compounds; administration & dosage; pharmacology; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Collagen Type I; metabolism; Dental Papilla; cytology; metabolism; Dose-Response Relationship, Drug; Drug Combinations; Female; Male; Oxides; administration & dosage; pharmacology; Rats; Rats, Sprague-Dawley; Silicates; administration & dosage; pharmacology
- From: Chinese Journal of Stomatology 2013;48(6):343-347
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of mineral trioxide aggregate(MTA) on the proliferation and differentiation of rat dental papilla cells(RDPC).
METHODSRDPC were cultured by tissue block method and identified.RDPC of the third passage were cultured with material extract fluid containing different mass concentrations of MTA (0.002, 0.02,0.2, 2 and 20 g/L) for 3 d, those cultured with routine culture fluid served as control group. The proliferation-related parameters were measured by methyl thiazolyl tetrazolium(MTT) assay. RDPC were cultured with material extract fluids containing 0.002 g/L MTA, those cultured with routine culture fluid served as control group, the activity of alkaline phosphatase(ALP) at 1, 3,5, 7 d and the level of collagen I at 1, 3,5 d were detected.
RESULTSMTT results showed that the A value of RDPC of group 20 g/L (0.092 ± 0.011) was less than that of the control group (0.249 ± 0.006) at 3 d(P < 0.01), the A value of RDPC of group 0.02 g/L (0.267 ± 0.005) and 0.002 g/L (0.276 ± 0.006) were more than that of the control group (0.249 ± 0.006) at 3 d(P < 0.01). ALP detection proved that ALP activity of MTA at 3 d (0.217 ± 0.008), 5 d (0.253 ± 0.005) , 7 d (0.279 ± 0.004) were more than that of the control group at 3 d (0.166 ± 0.006) ,5 d (0.221 ± 0.006), 7 d (0.242 ± 0.004) (P < 0.01). Collagen I detection showed that the level of collagen I of MTA at 3 d[(78.46 ± 2.72) µg/L], 5 d[(90.73 ± 3.08) µg/L] were more than that of the control group at 3 d[ (66.75 ± 3.08) µg/L], [5 d (74.27 ± 3.50) µg/L] (P < 0.01).
CONCLUSIONSMTA of high concentrations can significantly inhibit cell growth, and of low concentrations can promote cells proliferation and differentiation.
