Effect of mineral trioxide aggregate on proliferation and differentiation of rat dental papilla cells
10.3760/cma.j.issn.1002-0098.2013.06.006
- VernacularTitle:三氧化矿物凝聚体对牙乳头细胞增殖和分化影响的实验研究
- Author:
Fei-Lai LI
1
;
Guo-Ying QUE
Author Information
1. 中南大学湘雅医院口腔医学中心
- Keywords:
Cell proliferation;
Cell differentiation;
Dental papilla;
Mineral trioxide aggregate
- From:
Chinese Journal of Stomatology
2013;48(6):343-347
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of mineral trioxide aggregate (MTA) on the proliferation and differentiation of rat dental papilla cells(RDPC).Methods RDPC were cultured by tissue block method and identified.RDPC of the third passage were cultured with material extract fluid containing different mass concentrations of MTA (0.002,0.02,0.2,2 and 20 g/L) for 3 d,those cultured with routine culture fluid served as control group.The proliferation-related parameters were measured by methyl thiazolyl tetrazolium(MTT) assay.RDPC were cultured with material extract fluids containing 0.002 g/L MTA,those cultured with routine culture fluid served as control group,the activity of alkaline phosphatase(ALP) at 1,3,5,7 d and the level of collagen Ⅰ at 1,3,5 d were detected.Results MTT results showed that the A value of RDPC of group 20 g/L(0.092 ±0.011) was less than that of the control group(0.249 ±0.006)at 3 d (P ＜ 0.01),the A value of RDPC of group 0.02 g/L (0.267 ± 0.005) and 0.002 g/L (0.276 ±0.006) were more than that of the control group (0.249 ± 0.006) at 3 d (P ＜ 0.01).ALP detection proved that ALP activity of MTA at 3 d (0.217 ± 0.008),5 d (0.253 ± 0.005),7 d (0.279 ± 0.004) were more than that of the control group at 3 d(0.166±0.006),5 d(0.221 ±0.006),7 d(0.242 ±0.004)(P ＜0.01).Collagen Ⅰ detection showed that the level of collagen Ⅰ of MTA at 3 d[(78.46 ±2.72) μg/L],5 d [(90.73 ± 3.08) μg/L] were more than that of the control group at 3 d [(66.75 ± 3.08) μg/L],[5 d(74.27 ± 3.50) μg/L] (P ＜ 0.01).Conclusions MTA of high concentrations can significantly inhibit cell growth,and of low concentrations can promote cells proliferation and differentiation.
