Microscopic Raman spectral characteristics and diagnostic value of pathological lip minor salivary glands affected in primary Sj(o)gren's syndrome

VernacularTitle:原发性舍格伦综合征病变唇腺的显微拉曼光谱特征及诊断价值
Author: Li-Li XUE ¹ ; Dong-Chen OU ; Pei-Qiong CHEN ; Mei-Qing CHEN ; Pei SUN
Author Information

1. 厦门大学附属第一医院口腔科
Keywords: Sj(o)gren's syndrome; Salivary Glands,Minor; Spectrum Analysis,Raman
From: Chinese Journal of Stomatology 2013;48(8):477-480
CountryChina
Language:Chinese
Abstract: Objective To investigate the Raman spectral characteristics of the pathological lip minor salivary glands affected in primary Sjtǒgren's syndrome.Methods Thirty pathological samples and 30 normal samples were collected in this study.The samples were examined by Raman microscope.Support vector machine (SVM) was employed to analyze the data and establish the classification model.Results The spectra of pathological tissues was different from the controls in proteins,nucleic acids,lipids and glycogen skeleton.The sensitivity,specificity and accuracy of the model established by SVM on the training sets were all 92.0％ (92/100),but the sensitivity,specificity and accuracy of the model established by SVM on the testing sets were 69.2％ (37/53),100.0％ (37/37) and 82.0％ (73/89) respectively.Conclusions There was significant difference in Raman spectra between the pathological and normal lip salivary glands,and the classification model established by SVM could discriminate the pathological glands from the normal ones.