Effect of different stress conditions on growth and biofilm formation capability of Enterococcus faecalis
10.3760/cma.j.issn.1002-0098.2013.09.004
- VernacularTitle:不同理化条件对粪肠球菌生长周期及生物膜形成能力的影响
- Author:
Shu-Jun RAN
1
;
Jia E
;
Cai-Lian ZHU
;
Zhi-Yan HE
;
Jing-Ping LIANG
Author Information
1. 200011,上海交通大学医学院附属第九人民医院口腔医学院牙体牙髓科上海市口腔医学研究所上海市口腔医学重点实验室
- Keywords:
Enterococcus faecalis;
Biofilms;
Growth curve;
Stress conditions
- From:
Chinese Journal of Stomatology
2013;48(9):529-534
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of growth and biofilm formation capability of Enterococcus faecalis (Ef) in different stress conditions.Methods The changes of growth of Ef in stress conditions were observed by measuring the A600 value with ultraviolet spectrophotometer.Ef was incubated on glass slide in stress conditions,biofilm formation capability of cells was investigated by colony-forming unit (CFU) counting of the culturable bacteria and fluorescence confocal laser scanning microscopy.Results Ef couldn't growth under the conditions of 2％,5％ NaClO,pH =11 and 12,the A600 value was unchanged in 96 hours.But the growth curve changed at different levels in other stress conditions:under 1％ NaClO,the A600 value peaked at 1.461 at 16 hour(the peaked level was 1.238 at 6 hours in control group) ; under 0,0.05％,0.15％ glucose,it peaked at 0.645,0.890,1.173,respectively,at 6 hour (it was maximized to 1.195 at 6 hours in control group);the A600 value peaked at 1.704 at 6 hours at pH =9 and 1.225 at 10 hours at pH =10(the peak level was 1.732 at 6 hours at pH =7).Biofilm assay showed that Ef were able to form biofilm in these stress conditions except 5％ NaClO and pH =12.Conclusions Ef could growth and form biofilms in energy starvation,low concentrations of sodium hypochlorite and weak alkaline stress.
