Relationship between P-glycoprotein function in peripheral blood cells and multidrug resistance in breast carcinoma.
- Author:
Fei MA
1
;
Yu-qian LIAO
;
Ying FAN
;
Ying-hong WANG
;
Jian-ming LIANG
;
Jie MA
;
Bing-he XU
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; blood; Adult; Aged; Anthracyclines; administration & dosage; Antineoplastic Combined Chemotherapy Protocols; therapeutic use; Breast Neoplasms; drug therapy; metabolism; pathology; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Female; Humans; Killer Cells, Natural; metabolism; Middle Aged; Retrospective Studies; Rhodamine 123; metabolism; Taxoids; administration & dosage
- From: Chinese Journal of Oncology 2010;32(7):529-532
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the relationship between P-glycoprotein function in peripheral blood cells and primary multidrug resistance in breast carcinoma.
METHODSP-gp function was investigated by flow cytometry in NK cells of 16 breast cancer patients treated with anthracyclines and taxanes. Among all the patients, 8 were in chemotherapy-sensitive group and 8 in chemotherapy-resistant group. P-gp function was determined by rhodamine 123 (Rh123)-ejection test. Mathematical model was established by a regression of the fluorescence-time curve. The efflux rate constants of the chemotherapy-sensitive and -resistant groups were compared.
RESULTSThere was no significant difference of Rh123 accumulation, retention or efflux between the two groups. The mathematical model of F(t) = F(0) · e(-kt) was established. K was the efflux rate constant, which was significantly different between the chemotherapy-sensitive and -resistant groups (P = 0.025). When k > 3.9 was used as diagnostic criterium for primary resistance, the sensitivity, specificity and accuracy were 75.0%, 100% and 87.5%, respectively.
CONCLUSIONP-glycoprotein function in peripheral blood cells is associated with primary multidrug resistance in breast carcinoma. The efflux rate constant may be a good predictor for chemotherapy sensitivity.
