Significance of TCR gene clonal rearrangement analysis in diagnosis of mycosis fungoides.
- Author:
Chen XU
1
;
Yuan TANG
;
Lin WANG
;
Chuan WAN
;
Wei-ping LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Antigens, CD7; metabolism; Base Sequence; CD2 Antigens; metabolism; CD3 Complex; metabolism; CD4 Antigens; metabolism; Child; Child, Preschool; Female; Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; Humans; Leukocyte Common Antigens; metabolism; Male; Middle Aged; Molecular Sequence Data; Mycosis Fungoides; diagnosis; genetics; metabolism; pathology; Paraffin Embedding; Receptors, Antigen, T-Cell, alpha-beta; genetics; Receptors, Antigen, T-Cell, gamma-delta; genetics; Skin Neoplasms; diagnosis; genetics; metabolism; pathology; Young Adult
- From: Chinese Journal of Oncology 2010;32(9):685-689
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the significance of detecting TCR gene clonal rearrangement in the diagnosis of mycosis fungoides (MF) and to optimize the primers used for detecting the TCR gene clonal rearrangement with PCR in paraffin embedded tissues of MF.
METHODSNineteen cases of MF were enrolled into the study. A panel of 10 antibodies were used for immunophenotypic analysis and polymerase chain reaction for TCR-γ and TCR-β gene rearrangement detection in this study.
RESULTSTCR gene clonal rearrangements were detected in all 19 cases, in which 84.2% cases (16/19) had TCR-γ gene clonal rearrangements. The positive rates of the primers T(VG)/T(JX), V(2-5)/V(8-12)/JGT(1) and BIOMED-2-TCR-γ were 47.4%, 78.9% and 31.6%, respectively. The positive rate of V(2-5)/V(8-12)/JGT(1) was statistically significantly higher than that of T(VG)/T(JX) and BIOMED-2-TCR-γ (P < 0.05). No TCR gene clonal rearrangement was detected using the primers V(γ11)/V(γ101)/Jγ12 and V(γ11)/V(γ101)/J(p12). TCR-β gene clonal rearrangement was detected in 31.6% (6/19) cases.
CONCLUSIONSTCR gene clonal rearrangement analysis is a useful tool in the diagnosis of MF and TCR-γ gene is a good target gene for the detection. The primers T(VG)/T(JX), V(2-5)/V(8-12)/JGT(1) and BIOMED-2-TCR-γ can be used in clinicopathologic detection for TCR gene clonal rearrangement and V(2-5)/V(8-12)/JGT(1) may be the first choice.
