Bufalin induces apoptosis in osteosarcoma U-2OS and U-2OS methotrexate 300-resistant cell lines in vitro.
- Author:
Jin WANG
1
;
Jun-qiang YIN
;
Qiang JIA
;
Jing-nan SHEN
;
Gang HUANG
;
Xian-biao XIE
;
Chang-ye ZOU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Bone Neoplasms; metabolism; pathology; Bufanolides; pharmacology; Cell Cycle; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Drug Resistance, Neoplasm; Humans; Methotrexate; pharmacology; Osteosarcoma; metabolism; pathology; Proto-Oncogene Proteins c-bcl-2; metabolism; Tetrahydrofolate Dehydrogenase; metabolism; Tumor Suppressor Protein p53; metabolism; bcl-2-Associated X Protein; metabolism
- From: Chinese Journal of Oncology 2010;32(10):734-738
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the growth inhibition and apoptosis induction effects of bufalin on human osteosarcoma cell lines in vitro.
METHODSU-2OS and U-2OS/methotrexate (MTX) 300-resistant cell lines were treated with bufalin. Cell viability was assessed by MTT assay. Cell-cycle status, apoptosis-inducing effects, and the expression of apoptosis-related proteins were evaluated by flow cytometry, fluorescent staining, DNA fragmentation assay, and Western blotting.
RESULTSBufalin inhibited cell growth in both U-2OS and U-2OS/MTX300 cells. The IC(50) values of bufalin for U-2OS and U-2OS/MTX300 cells were (8.49 ± 2.1) ng/ml and (10.19 ± 1.7) ng/ml, respectively. The induction of G(2)/M cell-cycle arrest was also seen in the bufalin-treated cells. The bufalin-induced apoptosis was confirmed by increased expression of tumor suppressor protein p53, bax and decreased expression of bcl-2.
CONCLUSIONBufalin inhibits the growth of and induces apoptosis in both MTX-sensitive and MTX-resistant human osteosarcoma U-2OS cell lines. The apoptosis-inducing effect of bufalin is not influenced by the presence of high levels of DHFR.
