Cytotoxicity of allogenetic natural killer cells against CD34+ acute myelogenous leukemia cells.
- Author:
Xin-qing NIU
1
;
Kun-yuan GUO
;
Jian ZHOU
;
Liang-shan HU
;
San-fang TU
;
Miao-rong SHE
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD34; immunology; Coculture Techniques; Cytotoxicity, Immunologic; Flow Cytometry; Humans; K562 Cells; Killer Cells, Natural; immunology; Leukemia, Myeloid, Acute; immunology
- From: Journal of Southern Medical University 2008;28(2):173-175
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the cytotoxic effect of allogenetic natural killer (NK) cells in vitro on human CD34+ acute myelogenous leukemia cells.
METHODSCD34 expression on acute myelogenous leukemia KG1a cells was detected by flow cytometry. KG1a cells were co-cultured at different effector-to-target (E:T) ratios with NK cells isolated from 5 healthy individuals using magnetic cell sorting. Lactate dehydrogenase (LDH) release assay was employed to examine the cytolysis of KG1a cells in the co-culture, and the inhibition rate of the KG1a cell colony formation in methylcellulose was determined with K562 cells sensitive to NK cells as the control.
RESULTSA expression rate as much as (98.0-/+1.1)% was detected for CD34 antigen on KG1a cells, and the isolated NK cells (CD3(-)CD16+CD56+ cells) had a purity of (93.2-/+3.7)% after magnetic cell sorting. Allogenetic NK cells exhibited obvious cytotoxicity and colony inhibition in vitro against KG1a cells at different E:T ratios, and the effects were significantly enhanced as the E:T ratios increased (P<0.05). At the same E:T ratio, the cytotoxicity and colony inhibition rate of allogenetic NK cells against KG1a cells was lower than those against K562 cells (P<0.05).
CONCLUSIONAllogenetic NK cells exhibit obvious cytotoxicity and colony formation against CD34+ acute myelogenous leukemia cells.
