Association of dendritic cell distribution with intima hyperplasia in rats with diabetes mellitus.
- Author:
Qiang FU
1
;
Zhi-liang LI
;
Xiao-hua FU
;
Xiao LEI
;
Fei MIAO
;
Hong-chao WU
;
Ying-feng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Dendritic Cells; cytology; Diabetes Mellitus, Experimental; complications; pathology; Hyperplasia; complications; pathology; Rats; Rats, Sprague-Dawley; Spleen; cytology; Streptozocin; T-Lymphocytes; cytology; Tunica Intima; pathology
- From: Journal of Southern Medical University 2008;28(2):196-199
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association of dendritic cell distribution in the peripheral blood, spleen and arterial wall with intimal hyperplasia in rats with diabetes mellitus.
METHODSDiabetes mellitus was induced in rats by intraperitoneal injection of streptozotocin and high-fat feeding for 8 weeks. Peripheral blood, arterial wall and the spleen were collected from the rats to prepare cell suspensions, in which the proportions of dendritic cells and T cells were determined by flow cytometry.
RESULTSThe tunica intimal hyperplasia was more obvious in diabetic rats with or without high-fat feeding as compared with that of the control rats (P<0.05), and their dendritic cells decreased significantly in the peripheral blood (P<0.05) but increased in the arterial wall. The percentage of T cells was also increased in the peripheral blood and arterial wall of the diabetic rats.
CONCLUSIONChanges in the distribution of dendritic cells and T cells are closely associated with intimal hyperplasia in diabetic rats, suggesting the involvement of dendritic cells and T cells in the formation of atherosclerosis.
