Expression of survivin mRNA and protein in mitomycin-treated hepatoma carcinoma Hepa1-6 cells.
- Author:
Zhong-xin ZHOU
1
;
Ji-hong ZHANG
;
Long-juan ZHANG
;
Xiao-hui HUANG
;
Zheng-jun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; metabolism; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Humans; Inhibitor of Apoptosis Proteins; Liver Neoplasms; metabolism; Microtubule-Associated Proteins; metabolism; Mitomycin; pharmacology; RNA, Messenger; metabolism
- From: Journal of Southern Medical University 2008;28(2):230-232
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate survivin mRNA and protein expressions in mitomycin (MMC)-treated hepatoma carcinoma Hepa1-6 cells in vitro.
METHODSHepa1-6 cells were cultured in vitro in the presence of MMC at the concentrations of 1.0, 3.0 and 9.0 microg/ml, respectively, and 1 day and 3 days after the culture, the cell growth inhibition was assessed using MTT assay and the expressions of survivin were detected by RT-PCR and Western blotting.
RESULTSMMC at the concentration of 9.0 microg/ml resulted in significantly greater growth inhibition of the Hepa-6 cells than MMC at 1.0 and 3.0 microg/ml, and at the latter two concentrations, MMC treatment for 3 days did not produce obvious cell growth inhibition. Survivin expressions at both the mRNA and protein levels in Hepa1-6 cells were significantly decreased 1 day after MMC treatment at the 3 concentrations, and after 3-day MMC treatment at 1.0 and 3 microg/ml, survivin expressions increased to exceed the control level, whereas survivin maintained the low expression levels in cells treated with 9 microg/ml MMC for 3 days.
CONCLUSIONSurvivin expression in Hepa1-6 cells increases in response to MMC treatment at low doses, which might be one of the reasons for chemotherapeutic drug resistance.
