Effect of trichostatin A on proliferation and interleukin-2 expression of mouse T cells in mixed lymphocyte culture.
- Author:
Qiang WEI
1
;
Li-xin YU
;
Wen-feng DENG
;
Jun-sheng YE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Cell Survival; drug effects; Dose-Response Relationship, Drug; Enzyme Inhibitors; pharmacology; Female; Flow Cytometry; Histone Deacetylase Inhibitors; Hydroxamic Acids; pharmacology; Interleukin-2; biosynthesis; Lymphocyte Culture Test, Mixed; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; T-Lymphocytes; cytology; drug effects; metabolism
- From: Journal of Southern Medical University 2008;28(3):467-469
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of trichostatin A (TSA) on proliferation and interleukin-2 (IL-2) expression of mouse T cells in mixed lymphocyte culture (MLC), and explore its effect on T cell-mediated immune response.
METHODSBALB/c and C57BL mouse MLC was treated with different concentrations of TSA for different durations, and the lymphocyte inhibition ratio was measured by MTT assay. With CTX as the control, one-way MLC system of BALB/c and C57BL mouse was treated with the same concentrations of TSA, and IL-2 expression in the T cells was observed by flow cytometry.
RESULTSTSA inhibited the proliferation of T cells in the MLC in a time- and dose-dependent fashion. It also reduced the IL-2 expression in one-way MLC dose-dependently, showing it significantly differed from the effect of CTX (P<0.01).
CONCLUSIONHistone deacetylase inhibitor TSA can inhibit the proliferation and reduce IL-2 expression of the T cells in MLC of mice, and therefore inhibit the T cell-mediated immune response.
