Stabilizing the bactericidal activity of hydrogen peroxide: a brand new function of certain Chinese herbs.
- Author:
Wan-zhen LI
1
;
Ling-ling TAN
;
Qi-jie LI
;
Bang-jing ZHOU
;
Yong-xiang GAO
;
Wei-jun DING
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Bacterial Agents; pharmacology; Drugs, Chinese Herbal; pharmacology; Escherichia coli; drug effects; Hydrogen Peroxide; pharmacology; Microbial Sensitivity Tests; Staphylococcus aureus; drug effects; Sterilization; Time Factors
- From: Chinese journal of integrative medicine 2014;20(6):468-473
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore natural herbs to maintain the bactericidal activity of hydrogen peroxide (H).
METHODSEighteen extracts of Chinese herbs were prepared complying with the standard protocol. Each of the solutions was then mixed with 1% H2O2. The mixtures were handled with two approaches: autoclaved daily for one, two or three times; stored at room temperature from one through five years. Then the bactericidal activity were evaluated by assaying the minimal bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) against Gram positive (Staphylococcus aureus, ATCC25923) and Gram negative (Escherichia coli, ATCC12421) bacteria.
RESULTSWhile mixed with 1% H2O2, 10 out of 18 kinds of assessed Chinese herbs displayed MBC values at 1:12800 or higher after three times of autoclaving, and 8 of them preserved such level of MBC value after stored at room temperature for three years. Some Chinese herbs, i.e. R. Scutellariae, R. Coptidis, R. Bupleuri, H. Epimedii, C. Phelledendri and F. Chrysanthemi, can significantly maintain the bactericidal activity of diluted H2O2.
CONCLUSIONSCertain Chinese herbs can effectively stabilize the bactericidal activity of H2O2 undergoing autoclave or long-term storage. This paper reported a brandnew pharmaceutical function of Chinese herbs and provided experimental data for the potential enhancement of H2O2 usage while its stability level is promoted.
