Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B.
- Author:
Ze-long JIAO
1
;
Lei LI
;
Zi-gang ZHAO
;
Dan LIU
;
Bi-wen LIN
;
Heng-jin LI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Nucleus; drug effects; metabolism; Cell Survival; drug effects; Cytosol; drug effects; metabolism; Epithelial Cells; drug effects; enzymology; metabolism; Gene Expression Regulation; drug effects; Humans; I-kappa B Proteins; metabolism; Interleukin-6; genetics; metabolism; Interleukin-8; genetics; metabolism; Intracellular Space; drug effects; metabolism; Lipopolysaccharides; pharmacology; Mitogen-Activated Protein Kinases; metabolism; NF-KappaB Inhibitor alpha; NF-kappa B; metabolism; Ocimum; chemistry; Phosphorylation; drug effects; Plant Extracts; pharmacology; Protein Transport; drug effects; Proto-Oncogene Proteins c-akt; metabolism; RNA, Messenger; genetics; metabolism; Reactive Oxygen Species; metabolism; Respiratory System; cytology; Water
- From: Chinese journal of integrative medicine 2013;19(10):741-748
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the antiinflammatory activities of aqueous extract of Occimum gratissmium (OGE) with emphasis on expression of proinflammatory cytokines in Lipopolysaccharide (LPS)-stimulated epithelial cell BEAS-2B.
METHODSEffects of OGE on cell viability were determined by MTT assay. mRNA expression were analyzed by and reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. Activation of kinase cascades was investigated by immunoblot. Intracellular reactive oxygen species (ROS) was analyzed by flow cytometry.
RESULTSOGE (<200 μg/mL) treatment or pretreatment and following LPS exposure slightly affected viability of BEAS-2B cells. Increase of interleukin (IL)-6 and IL-8 and the elevated level of intracellular ROS in LPS-stimulated BEAS-2B cells were diminished by OGE pretreatment in a dose-dependent manner. OGE suppressed inflammatory response-associated mitogen-activated protein kinases (MAPKs) and Akt activation. Additionally, OGE pretreatment increased level of cellular inhibitor of κBα (IκBα) and inhibited nuclear translocation of nuclear factor kappa B (NF-κB).
CONCLUSIONThese findings indicate that significant suppression of IL-6 and IL-8 expressions in LPS-stimulated BEAS-2B cells by OGE may be attributed to inhibiting activation of MAPKs and Akt and consequently suppressing nuclear translocation of NF-κB.
