Screen for natural benzylisoquinoline alkaloids against tumor.
- Author:
Zhengfu ZHANG
1
;
Ying GUO
;
Xionghui WEI
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; analysis; isolation & purification; pharmacology; Antineoplastic Agents, Phytogenic; pharmacology; Benzylisoquinolines; analysis; isolation & purification; pharmacology; Cell Line, Tumor; Cell Proliferation; drug effects; Chelidonium; chemistry; Coptis; chemistry; Drug Evaluation, Preclinical; Drugs, Chinese Herbal; analysis; isolation & purification; pharmacology; Humans; Neoplasms; drug therapy; physiopathology; Papaveraceae; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(19):2684-2688
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo search for natural benzylisoquinoline alkaloids against tumor.
METHODIn this study, taken DNA as target, a method was developed for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine by the use of centrifugal ultrafiltration combined with HPLC technology. The anti-tumor activity for the compounds screened was evaluated in vitro.
RESULTNine compounds interacted with DNA were discovered and identified from Macleaya cordata, Chelidonium majus, Coptis chinensis, and the proliferation of four types of human solid cancer cell lines was markedly inhibited by these compounds.
CONCLUSIONThe developed method was considered to be suitable for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine.
