Study on effect of berberine on modulating lipid and CPT I A gene expression.
- Author:
Hong WANG
1
;
Lingyun SHI
;
Huafeng YIN
;
Qixin ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Berberine; administration & dosage; Carnitine O-Palmitoyltransferase; genetics; metabolism; Disease Models, Animal; Drugs, Chinese Herbal; administration & dosage; Gene Expression; drug effects; Humans; Hyperlipidemias; drug therapy; enzymology; genetics; metabolism; Lipid Metabolism; drug effects; Male; PPAR gamma; genetics; metabolism; Random Allocation; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2011;36(19):2715-2718
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the modulating effect on lipid and gene expressions of CPT I A caused by berberine (Ber) in experimental hyperlipidemia rats.
METHODMale SD rats were randomly divided into 5 groups according to the blood lipid values: normal group, hyperlipidemia group, 300 mg x kg(-1) x d(-1) Ber-treated group, 60 mg x kg(-1) x d(-1) Ber-treated group, and 7.2 mg x kg(-1) x d(-1) lovastatin-treated group. Normal group were fed with base diet and other groups were fed with high fat and cholesterol diet. 12 weeks after drugs were given the TC, TG, LDL-C, and HDL-C from rat blood samples were tested by automatic biochemistry analyzer. Gene expressions of CPT I A and PPARalpha were evaluated by RT-PCR and Western blot, respectively.
RESULTIt was shown that Ber significantly decreased TC and LDL-C, but increased HDL-C in dose-dependent manner, elevated expressions of CPT I A mRNA and protein without influence on PPARalpha expression. Similar effects from lovastatin on lipidemia were observed except the Ber effect on CPT I A gene expression.
CONCLUSIONBer has modulating effect on the lipid metabolism, the mechanism of which may be by promoting the CPT I A gene expression.
