Simultaneous determination of three sesquiterpene lactones in Inula hupehensis by RP-HPLC.
- Author:
Xiaolei WANG
1
;
Xianghe LI
;
Yi GUO
;
Jinlong QI
;
Linfei SU
;
Yan FU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Inula; chemistry; metabolism; Lactones; analysis; Sesquiterpenes; analysis
- From: China Journal of Chinese Materia Medica 2011;36(18):2520-2524
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA RP-HPLC method was developed for simultaneous determination of bigelovin, ergolide and tomentosin in Inula hupehensis.
METHODAn Agilent C18 column (4.6 mm x 250 mm, 5 microm) was used for separation at 40 degrees C. The mobile phase was acetonitrile-water, and the flow rate was 1.2 mL x min(-1). The detection wavelength was set at 210 nm.
RESULTThe method has good linearity in the ranges of 0.01792-0.1792 g x L(-1) (r =0.9999) for bigelovin, 0.0424-0.4240 g x L(-1) (r =0.9996) for ergolide, and 0.044 8-0.4480 g x L(-1) (r = 0.9996) for tomentosin. The average recoveries of bigelovin, ergolide, and tomentosin were 98.5%, 98.2%, 98.4%, with the RSD of 1. 3%, 1.3%, 1.7%, respectively. The results demonstrated that there was a significant difference in the contents of three sequterpene lactones among the tested Inulae Flos.
CONCLUSIONThe results indicated that the present RP-HPLC method is simple, quick and accurate, and can be used for the quality control of I. hupehensis, especially for the authentication of Inulae Flos.
