Study on HPLC chromatographic fingerprint of anti-tumor active site SSCE of Caulis spatholobi.
- Author:
Hong WANG
1
;
Yina LIU
;
Zuping ZENG
;
Wei HE
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; analysis; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; chemistry; Fabaceae; chemistry; metabolism; Flavonoids; analysis; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2011;36(18):2525-2529
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the chromatographic fingerprints for the anti-tumor flavonoids of Caulis spatholobi (SSCE). It could used to reflect the chemical information in this part comprehensively, and identify the chemical consitituents preliminarily.
METHODThe HPLC-DAD analysis method was performed on the column Kromasil 100-5PHENYL (4.6 mm x 250 mm, 5 microm). The mobile phase was water (0.5% acetic acid)- methanol in gradient elution and the detection wavelength was 254 nm.
RESULTThe chromatographic fingerprint of SSCE was established, which showed 16 characteristic peaks from 10 batches of medicinal materials. Among them, the peaks 1, 3, 4, 5, 8, 9, 10, 12, 13, and 16 were identified 3,4-dihodroxybenzoic acid, 4-Hydroxybenzoic Acid, epicatechin, puerarin, daidzein, liquiritigenin, calycosin, genistein, formononetin, and prunetin, respectively.
CONCLUSIONThe method is convenient, reproducibility and stability. It can used for quality control of the anti-tumor flavonoids of C. spatholobi (SSCE).
