Studies on influence of Siwu decoction and its composite drugs on chemical-induced blood-deficiency model mice.
- Author:
Min ZHU
1
;
Jin'ao DUAN
;
Yuping TANG
;
Shulan SU
;
Yongqing HUA
;
Jianming GUO
;
Dawei QIAN
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine Triphosphatases; drug effects; metabolism; Angelica sinensis; Animals; Bone Marrow Cells; drug effects; metabolism; pathology; Cell Cycle; drug effects; Cyclophosphamide; Disease Models, Animal; Drugs, Chinese Herbal; metabolism; pharmacology; Female; Glucosephosphate Dehydrogenase Deficiency; chemically induced; drug therapy; metabolism; pathology; Liver; drug effects; metabolism; Mice; Mice, Inbred ICR; Paeonia; Phenylhydrazines; Protective Agents; pharmacology; Spleen; drug effects; metabolism
- From: China Journal of Chinese Materia Medica 2011;36(18):2543-2547
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate Siwu decoction and its composite drugs on the blood-deficiency model mice induced by acetylphenyhydrazine and cyclophosphamide.
METHODAcetylphenyhydrazine and cyclophosphamide were used to copy the blood-deficiency model mice. Automatic hematology analyzer was used to test the peripheral hemogram. Weighting method was used to test the liver index and spleen index; Kits for ATPase test was used to test the activities of Na+ - K+ - ATPase/Ca2+ - Mg2+ - ATPase in erythrocyte membrane. Flow cytometry was used to test the bone marrow cells' cell cycle.
RESULTAngelicar Sinensis Radix and Paeoniae Radix Alba had the most effective activity on the peripheral hemogram. Paeoniae Radix Alba, the drug pair including Angelicar Sinensis Radix and the drug- group including Paeoniae Radix Alba had the most effective activity on the liver index. All the drugs, drug-pairs, drug-groups and the formula had effect on the spleen index. To the activity of Na+ - K+ - ATPase in erythrocyte membrane, Rehmanniae Radix Praeparata, the drug-pairs and drug-groups including Angelicar Sinensis Radix exhibited the most effective activity. All the drugs, drug-pairs, drug-groups and the formula had the protective effect on the damaged bone marrow cells.
CONCLUSIONSiwu decoction and its composite drugs all had effect on the blood-deficiency model mice, but the action intensity was different. Angelicar Sinensis Radix and Paeoniae Radix Alba exhibited the most effective activity to the protection of the blood-deficiency model mice.
