Anti-HIV activity and mechanism of Cynanchum otophyllum glucan sulfate in vitro.
- Author:
Jian TAO
1
;
Jing YANG
;
Chaoyin CHEN
;
Shenglan ZHAO
;
Kunlong BEN
Author Information
- Publication Type:Journal Article
- MeSH: Anti-HIV Agents; pharmacology; Cell Fusion; Cell Line; Cell Proliferation; drug effects; Cynanchum; chemistry; Glucans; isolation & purification; pharmacology; HIV-1; drug effects; Humans; Inhibitory Concentration 50; Plant Extracts; pharmacology; Plant Roots; chemistry; metabolism; Viral Envelope Proteins; drug effects
- From: China Journal of Chinese Materia Medica 2011;36(18):2548-2551
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study anti-HIV activity and mechanism of Cynanchum otophyllum glucan sulfate in vitro.
METHODAnti-HIV-1 activity was detected with syncytial formation assay and quantitative P24 enzyme-linked immunosorbent assay (ELISA); cytotoxicity was tested with MTT colorimetric assay. Antiviral mechanism was investigated by fusion inhibition, time of addition and pre-treatment experiments.
RESULTThe 50% inhibition concentrations (IC50) of PS20 for HIV-1(IIIB), HIV-1(Ada-M), and HIV-1(Bal), were 0.26, 0.46, 0.90 micromol x L(-1), respectively. Studies on antiviral mechanism of PS20 showed that target molecule may be viral envelope protein.
CONCLUSIONThe results suggested that PS20 had high anti-HIV activity and was worth to be studied further.
