Research on mechanism of energy metabolism disorders of rat's hepatoxicity induced by saikosaponins.
- Author:
Rongmei WANG
1
;
Lili LV
;
Wei HUANG
;
Youyi HUANG
;
Rong SUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antimetabolites; toxicity; Chemical and Drug Induced Liver Injury; metabolism; pathology; Disease Models, Animal; Dose-Response Relationship, Drug; Electron Transport; drug effects; Energy Metabolism; drug effects; Female; Liver; enzymology; pathology; Male; Mitochondria, Liver; drug effects; metabolism; Oleanolic Acid; analogs & derivatives; toxicity; Organ Size; drug effects; Rats; Saponins; toxicity
- From: China Journal of Chinese Materia Medica 2011;36(18):2557-2561
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the influence of saikosaponins on function of rats' liver mitochondria, its liver damage mechanism was discussed.
METHODAdministrating alcohol eluent of saikosaponins of different dose for 15 days to rats, and the high, middle and low lose-group are separately 300, 150, 50 mg x kg(-1) caculated by total saikosaponins. The liver index in serum, the respiratory function of liver mitochondria,the content of ATP and the activity of ATP enzyme were detected. The weight of heart, liver, spleen, lung, renal of rats were precisionly weighed, and the ratio of organ to body were calculated. The histopathologic examination of hepatic tissue were examined.
RESULTAlcohol eluent of saikosaponins of different dose can induce apparent decrease of PCR, P/O value, respiratory oxygen consumption and the activity of ATP enzyme; the level of ALT, AST and ALB in serum increased; the liver weight and the ratio of liver to body increaseed, and the hepatic tissue damage is obvious in the histopathologic examination of hepatic tissue. The above-mentioned changes gradually aggravates with dose increasing, and it is obviously discrepancy compared with control group.
CONCLUSIONAlcohol eluent of saikosaponins can induce liver damage by restraining the respiratory function of mitochondria and effecting liver's energy metabolism. Other hepatoxicity mechanism still need to be discussed.
