Effect of zibuganshenfang on the expression and activity of tyrosinase on B16 cells.
- Author:
Jingxia ZHAO
1
;
Lei ZHANG
;
Ping LI
;
Xin LIU
;
Yan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; pharmacology; Medicine, Chinese Traditional; methods; Melanoma, Experimental; enzymology; Mice; Monophenol Monooxygenase; drug effects; Tumor Cells, Cultured; Vitiligo; drug therapy; enzymology
- From: China Journal of Chinese Materia Medica 2011;36(18):2567-2570
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEZiBuGanShenFang (ZBGSF) is a traditional herbal formula, which has showed an outstanding therapeutic effect on vitiligo clinically. Our aim is to investigate the influence of ZBGSF drug serum on the expression and activity of Tyrosinase in B16 cells, explore the mechanism of ZBGSF on Vitiligo treatment further.
METHODtyrosinase activity was measured by zymological methods, western blotting and RT-PCR were used to measure the protein content and mRNA level of tyrosinase and related proteins, respectively.
RESULTZBGSF drug serum had no effect on the proliferation of B16 cells. But it could promote Tyrosinase activity significantly and increase protein content and mRNA level of tyrosinase and related proteins in B16 cells.
CONCLUSIONPromoting the expression of tyrosinase protein and mRNA may be the elementary basis of ZBGSF on Vitiligo treatment.
