Expression of coxsackievirus and adenovirus receptor in non-small cell lung cancer and its significance.
- Author:
An-kang GU
1
;
Lei-na SUN
;
Zhao-li CHEN
;
Zhong-li ZHAN
;
Jun-wen LI
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma, Bronchiolo-Alveolar; metabolism; pathology; Adult; Aged; Biomarkers, Tumor; metabolism; Blotting, Western; Carcinoma, Non-Small-Cell Lung; metabolism; pathology; Coxsackie and Adenovirus Receptor-Like Membrane Protein; Female; Humans; Lung; metabolism; Lung Neoplasms; metabolism; pathology; Lymphatic Metastasis; Male; Middle Aged; Prognosis; RNA, Messenger; metabolism; Receptors, Virus; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Oncology 2009;31(4):278-281
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mRNA and proten expression of coxsackievirus and adenovirus receptor (CAR) in the corresponding normal lung tissue, para-neoplastic tissue and lung cancer tissue, and the correlation of CAR expression with the carcinogenesis as well as the expression difference in various clinicopathologic parameters.
METHODSThe expression of CAR mRNA and protein in the samples from 32 lung cancer patients was determined by RT-PCR and Western blot, respectively.
RESULTSThe expression level of CAR mRNA and protein in normal lung tissue, paraneoplastic tissue and cancer tissue were 1.000 +/- 0.012, 1.048 +/- 0.035, 1.282 +/- 0.072, and 0.902 +/- 0.038, 0.944 +/- 0.042, 1.08 +/- 0.052, respectively, with a statistical significance among the groups (P = 0.022, P = 0.007, P = 0.009, P = 0.027). There was a statistically significant positive correlation between expression of CAR mRNA and that of CAR protein (r = 0.448, P = 0.026). The expression levels of CAR were significantly different among different pathological types (P = 0.012), with a high level of CAR in all 7 bronchiolo-alveolar carcinoma (BAC, P = 0.029). However, there was no statistical significance in other clinicopathologic parameters (P > 0.05), including gender, age, smoking or not, tumor size, with or without lymph node metastasis and TNM stage.
CONCLUSIONThe expression of CAR mRNA and protein in cancer tissue samples are significantly higher than that in the normal and paraneoplastic samples, indicating that CAR might play a crucial role in the carcinogenesis. It may become a new potential prognostic marker for lung cancer patients.
