MT1-MMP up-regulates VEGF expression in human breast carcinoma MCF-7 cells and induces tumor angiogenesis.
- Author:
Yi-ping DENG
1
;
Wei LI
;
Yi-lei LI
;
Hua XU
;
Shan-shan LIANG
;
Li-hong ZHANG
;
Yu-lin LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Breast Neoplasms; metabolism; pathology; Cell Line, Tumor; Female; Gene Expression Regulation, Neoplastic; Humans; Matrix Metalloproteinase 14; genetics; metabolism; physiology; Mice; Mice, Nude; Microvessels; pathology; Neoplasm Transplantation; Neovascularization, Pathologic; pathology; RNA, Messenger; metabolism; Tumor Burden; Up-Regulation; Vascular Endothelial Growth Factor A; genetics; metabolism
- From: Chinese Journal of Oncology 2009;31(10):727-731
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the function of MT1-MMP in tumor angiogenesis and elucidate the possible way of action that MT1-MMP contributes to angiogenesis.
METHODSMT1-MMP was transfected into human breast carcinoma cell line MCF-7 cells. Semi-quantitative RT-PCR and immunofluorescence staining were employed to detect the expression of VEGF in the transfected and non-transfected MCF-7 cells. Tumor growth, microvessel density and expression of VEGF in nude mice were detected through in vivo tumorigenicity assay.
RESULTSIn MT1-MMP stable transfected MCF-7 cells, mRNA expression of VEGF(189), VEGF(165), and VEGF(121) and immunofluorescence intensity were significantly elevated (P < 0.001). In vivo tumorigenicity assay in the nude mice showed that MT1-MMP promoted tumor growth. The MVD in the MT1-MMP-transfected cells-transplanted tumor tissue was significantly elevated (P < 0.05). Immunohistochemical assay showed that there was a strong immunostaining of VEGF in those tumor tissues.
CONCLUSIONMT1-MMP can induce tumor angiogenesis through up-regulation of VEGF expression. This function of MT1-MMP may open a new approach for clinical anti-tumor research and anti-tumor drug development.
