Expression of Pin1, beta-catenin and cyclin D1 in salivary adenoid cystic carcinoma and its significance.
- Author:
Chuan-xiang ZHOU
1
;
Yan GAO
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Adenoid Cystic; metabolism; pathology; Cell Line, Tumor; Cyclin D1; metabolism; Humans; Lymphatic Metastasis; NIMA-Interacting Peptidylprolyl Isomerase; Neoplasm Invasiveness; Peptidylprolyl Isomerase; metabolism; Salivary Gland Neoplasms; metabolism; pathology; beta Catenin; metabolism
- From: Chinese Journal of Stomatology 2006;41(10):623-626
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of Pin1, beta-catenin and cyclin D1 in salivary adenoid cystic carcinomas (SACC) and to evaluate the role of beta-catenin and Pin1 in SACC carcinogenesis.
METHODSThe expressions of Pin1, beta-catenin and cyclin D1 were examined in the specimens of 65 patients with SACC by immunohistochemistry, and Pin1 protein and mRNA expressions detected by Western blot and RT-PCR in four SACC cell lines.
RESULTSPin1 was overexpressed in 51 cases of ACC (78%), and 41 (63%) cases showed positive immunoreactivity for cyclin D1 protein in the nuclear fraction in tumor tissues. Fourteen (22%) cases showed positive immunoreactivity for beta-catenin protein in the nuclear/cytoplasmic fraction in tumor tissues, 6 of which exhibited quite evident expression of beta-catenin in nucleolus. The expression of membranous beta-catenin was down-regulated in most of the patients with lymph node metastasis (11/14).
CONCLUSIONSThe results suggest that Pin1 and beta-catenin signalling pathway were activated in SACC and might play a pivotal role in SACC carcinogenesis and metastasis.
