Effects of arecoline and nicotine on the expression of hTERT in oral keratinocytes.
- Author:
Yi-jun GAO
1
;
Tian-you LING
;
Xiao-min YIN
;
Xia LI
;
Yan HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Arecoline; pharmacology; Cells, Cultured; Cholinergic Agonists; pharmacology; Dose-Response Relationship, Drug; Drug Synergism; Ganglionic Stimulants; pharmacology; Humans; Keratinocytes; drug effects; enzymology; Mouth Mucosa; enzymology; pathology; Nicotine; pharmacology; RNA, Messenger; genetics; metabolism; Telomerase; genetics; metabolism
- From: Chinese Journal of Stomatology 2007;42(1):26-30
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of arecoline and nicotine on the expression of human telomerase reverse transcriptase (hTERT) mRNA and protein in cultured normal human oral keratinocytes (KC).
METHODSThe experiments were divided into arecoline group, arecoline/nicotine group and control group. The hTERT mRNA and protein expression of KC was examined by reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
RESULTSArecoline could induce the hTERT mRNA and protein expression of KC in a dose dependent manner, the hTERT mRNA and protein expression of KC was higher in 0.030, 0.060, 0.090 g/L arecoline group than control group (P < 0.001). Nicotine (0.025 g/L) increased hTERT mRNA and protein expression of KC induced by arecoline.
CONCLUSIONSArecoline could increase the expression of hTERT mRNA and protein in oral keratinocytes. Nicotine had a synergistic effect on arecoline. hTERT over-expression induced by arecoline and nicotine may play an important role in the malignant transformation of oral submucous fibrosis.
